Monoclonal antibody to proteoglycan derived from Grifola frondosa (Maitake)

Hirata A, Adachi Y, Itoh W, Komoda M, Tabata K, Sugawara I

Research Laboratory, Taito Co., Ltd., Kobe, Japan.

A murine monoclonal antibody (MAb) was prepared by immunizing BALB/c mice with a
proteoglycan fraction derived from Grifola frondosa (Maitake mushroom), followed by the
hybridization of spleen cells with mouse myeloma cells. The MAb (subclass; Ig G2b), designated
MPG2, reacted with schizophyllan (SPG), curdlan, scleroglucan, laminarin and lentinan, but not
with dextran, pullulan, mannan and xylan. Immunohistochemistry (ABC-GO method) showed
that MAb MPG2 reacted with lysosomal proteoglycan and (1-->6)-beta-branched laminaritriose
taken up by rabbit peritoneal macrophages. These results suggest that this MAb may recognize
mainly (1-->3)-beta-D-glucan, and may be useful for determining the immunological properties of
Grifola frondosa-derived proteoglycan.

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