Flammulina velutipes AM-G15

Agrocybe aegerita AM-G11

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Strain: Flammulina velutipes AM-G15


Common Names: Winter Mushroom, Snow Puff (Peak), Velvet Foot, Enokitake (榎茸, エノキタケ), Jinzhengu (金針菇), Paengi Beoseot (팽이버섯), Collybie à Pied Velouté, Gemeiner Samtfußrübling, Winter Armillaria (Опёнок зи́мний)


=Agaricus velutipes Curtis


The enoki or enokitake, Flammulina velutipes is a popular mushroom, mostly being cultivated using the bottle method in Japan, China and Korea. The mushroom naturally grows on the stumps of the Chinese hackberry tree ("enoki" in Japanese), but also on other trees, such as mulberry and persimmon trees. There is a significant difference in appearance between the wild and cultivated types of the mushroom. Cultivated mushrooms have not been exposed to light, resulting in a white color, whereas wild mushrooms usually display a dark brown color. Cultivated mushrooms are grown in a carbon dioxide (CO2)-rich environment to nurture the development of long thin stems, whilst wild mushrooms produce a much shorter and thicker stem. This is a choice edible mushroom used in soups in traditional Japanese cuisine.


Application: choice edible, medicinal usage and compounds.


Characteristics: The cultivated strains have a different morphology than those collected in the wild. Small caps and long stems are achieved by elevating carbon dioxide levels.


Temperature Range: Low: 50-60 °F (10-16 °C) fruiting temperature; decrease from 70-75 °F (21-24 °C). High CO2 2-5000 ppm.


Recommended Substrate: hardwood sawdust (poplar, beech, oak, birch, etc), straw, corn cobs, and other agricultural wastes (cotton, coffee, paper, sugarcane baggase, etc).

Cellulose Decomposers: Hardwood Sawdust

Most popular species under cultivation are naturally wood inhabiting fungi. So, growing on sawdust is a logical choice. Sawdust is mixed with wheat bran (or another nitrogen source) at 5-15% and Calcium Carbonate (CaCO3) (a buffering agent) at 2-3%, and filled into autoclavable bags. The bags are sterilized for a minimum of 1 hour at 121°C (15 psi when at sea level). Note sterilization exposure times vary depending on pressure and elevation. Finally, the substrate is cooled to at least 25°C (approx. 80°F) then, inoculated with grain or liquid spawn and incubated at appropriate temp for the species until colonization is complete.

Recommended species on hardwood sawdust:

Agrocybe aergerita, Antrodia camphorata, Armillaria mellea, Auricularia auricular-judae, Fistulina hepatica, Flammulina velutipes, Fomes fomentarius, Ganoderma applanatum, G. australe, G. lucidum, G. curtisii, Grifola frondosa, Hericium americanum, H. clathroides, H. coralloides, H. erinaceus, Hypholoma capnoides, H. sublateritium, Hypsizygous marmoreus, H. tessulatus, H. ulmarius, Inonotus obliquus, Laetiporus sulphureus, Lentinula edodes, Macrolepiota procera, Omphalotus sp., Panellus stipticus, Phellinus linteus, Pholiota nameko, Piptoporus betulinus, Pleurotus sp., Polyporus squamosus, Polyporus umbellatus, Schizophylum commune, Sparassis crispa, Stropharia rugoso-annulata, Trametes sp., Tremella mesenterica, and Xylaria hypoxylon.


Recommended Media Type: PDY MYA MEP


History: from China, cultivated strain, 5.2005; S. P. Wasser; rec. 2011 on plate


DNA Sequence: DNA Sequencing for ITS region available at an extra charge of $200. Allow two weeks for the DNA sequencing service.

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Price: $225

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Overnight shipping is recommended for this culture

Our technical staff is available to answer your questions on culture media, culture maintenance, fruiting strategies or other questions that may arise as you work with cultures. For more details please see Consulting Services.


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