Lentinula edodes AM-P8

Agrocybe aegerita AM-G11

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Strain: Lentinula edodes AM-P8


=Lentinus edodes (Berk.) Singer


Common Names: Shiitake (シイタケ), Xiānggū (香菇), Pyogo (표고), Lentin des Chênes, Champignon parfumé, Шиитаке


Shiitake (Letinula edodes), are native to Japan, China and Korea and have been grown in all three countries for over one thousand years. Shiitake are saprophytic white-rot fungi by nature and thrive predominantly on the shii tree (Castanopsis cuspidata), fruiting solitary or in clusters. They have a tan to dark brown pileus, convex to plane shaped, with an incurved margin, measuring 5-25cm in diameter. Scales and cracking may be present depending on the strain and growing climate. They have white lamellae and spores. They also bear a thick white, fibrous, central stem, and a ring or annulus is absent. A pleasant nutty, almost sweet odor can be detected from mycelium and fruiting bodies, becoming stronger when dried.

The oldest record regarding the shiitake mushroom dates back to AD 199 at the time of Emperor Chūai in Japan. The first written record of shiitake cultivation can be traced to Wu Sang Kwuang in China, born during the Song Dynasty (AD 960–1127). During the Ming Dynasty (AD 1368–1644), physician Wu Juei wrote that the mushroom could be used not only as a food but as a medicinal mushroom, taken as a remedy for upper respiratory diseases, poor blood circulation, liver trouble, exhaustion and weakness, and to boost qi, or life energy. It was also believed to prevent premature aging. The Japanese cultivated the mushroom by cutting shii trees with axes and placing the logs by trees that were already growing shiitake or contained shiitake spores. Uninoculated logs would be soaked in water and hit with axes, and laid adjacent to fruiting logs. Fruiting bodies would sporulate and colonize the uninoculated logs. This is known as the soak and strike method. The Japanese perfected this cultivation method and produced over 80% of the world’s supply of shiitake from 1940-1986. In 1987, the Chinese developed bag culture and revolutionized mushroom cultivation to make it what it is today. Sawdust is placed into autoclavable bags, sterilized, and then inoculated with grain spawn. Bag culture allows mushrooms to be cultivated year-round, and brings the operation indoors, where the climate can be controlled to induce fruiting. Shiitake will fruit on a diversity of substrates. Typically non-aromatic hardwoods such as oak (Quercus spp.), Poplar (Populous spp.), and Alder (Alnus spp.) are used, however eucalyptus, straw, and pine supplemented with hardwood sawdust have also been used successfully. It is strain dependent on the ability to degrade different lignocellulosic compounds and some strains are better adapted to specific substrates than others. For more information on shiitake cultivation, consult the mushroom grower’s handbook 2 at https://www.alohamedicinals.com/book2/chapter-4-01.pdf.


Application: Our very best commercial shiitake strain AM-P8 is a choice edible with strong growth characteristics and outstanding shelf life. The cap is thick and meaty, and the stem is very thin with minimal trimming waste. This strain puts all its energy into growing caps - a great economic characteristic for commercial production. This is rapidly becoming the commercial strain of choice for growers around the world.


Characteristics: Typically two toned color with center dark and outer area lighter, very uniform size fruitbodies characterized by medium sized cap and thin stem. Absolutely our favorite commercial strain, with the most consistent harvest and the longest shelf life - up to 30 days refrigerated. This is a very hardy Shiitake strain that will out-compete most contaminants. Will readily fruit with no triggering, although a drop in temp or soaking the blocks also yields reliable flushes. Days pinning until harvest 6. Very typically over 100% Biological Efficiency.


Temperature Range: Mid: 50-75 °F (10-24 °C) fruiting temperature.


Recommended Substrate: sawdust (oak, chinkapin, tanoak, hornbeams, alder, aspen, poplar, cottonwood, beech, birch, chestnut, hickory, maple, sweetgum, tupelo, willow). Also cultivated on straw and conifer wood.

Cellulose Decomposers: Hardwood Sawdust

Most popular species under cultivation are naturally wood inhabiting fungi. So, growing on sawdust is a logical choice. Sawdust is mixed with wheat bran (or another nitrogen source) at 5-15% and Calcium Carbonate (CaCO3) (a buffering agent) at 2-3%, and filled into autoclavable bags. The bags are sterilized for a minimum of 1 hour at 121°C (15 psi when at sea level). Note sterilization exposure times vary depending on pressure and elevation. Finally, the substrate is cooled to at least 25°C (approx. 80°F) then, inoculated with grain or liquid spawn and incubated at appropriate temp for the species until colonization is complete.

Recommended species on hardwood sawdust:

Agrocybe aergerita, Antrodia camphorata, Armillaria mellea, Auricularia auricular-judae, Fistulina hepatica, Flammulina velutipes, Fomes fomentarius, Ganoderma applanatum, G. australe, G. lucidum, G. curtisii, Grifola frondosa, Hericium americanum, H. clathroides, H. coralloides, H. erinaceus, Hypholoma capnoides, H. sublateritium, Hypsizygous marmoreus, H. tessulatus, H. ulmarius, Inonotus obliquus, Laetiporus sulphureus, Lentinula edodes, Macrolepiota procera, Omphalotus sp., Panellus stipticus, Phellinus linteus, Pholiota nameko, Piptoporus betulinus, Pleurotus sp., Polyporus squamosus, Polyporus umbellatus, Schizophylum commune, Sparassis crispa, Stropharia rugoso-annulata, Trametes sp., Tremella mesenterica, and Xylaria hypoxylon


Recommended Media Type: PDY MYA MEP WMYA WMEP WMEP


History: from Ukraine; rec. in plate


DNA Sequence: DNA Sequencing for ITS region available at an extra charge of $200. Allow two weeks for the DNA sequencing service.

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Price: $350

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Overnight shipping is recommended for this culture

Our technical staff is available to answer your questions on culture media, culture maintenance, fruiting strategies or other questions that may arise as you work with cultures. For more details please see Consulting Services.


For all of you formulators - Aloha Medicinals also offers bulk powder ingredients for use in your formulations, which are 100% USDA and EU Certified Organic. Call our customer service representative at (775) 886-6300 for more information and to place your order today!